Duration of the elective: 1 month



Number of students: 1



Prerequisites: None



Location(s): Koç University Rumelifeneri Campus, Koç University Hospital (KUTTAM)









Elective Director: Prof. Dr. Tamer Önder



Elective Faculty: Nazlı Başak, Caner Süsal, Devrim Gözüaçık, Tuğba Bağcı Önder, Ceyda Açılan Ayhan, Yıldız Tütüncü, Gözde Korkmaz, Ece Öztürk, Zelal Adıgüzel, Tamer Önder








Medical Biology forms a bridge between basic research and clinical medicine. It is the cornerstone of modern healthcare, biomedical research, and advanced drug development.




This elective will introduce students to advanced cell and molecular biology techniques used in the research program of the faculty including, but not limited to, cancer biology, stem cell biology, tissue culture, cellular reprogramming, and genome editing.




Students who express interest in an ongoing research project by Medical Biology faculty will initially contact the PI, and if a mutual agreement is made, they can apply for the elective. This elective can be taken in the 5th or 6th year.









Students will formulate a novel hypothesis and test it with appropriate experimentation. Students will gain skills in understanding primary literature, planning experiments with appropriate controls, keeping careful records.





Specific objectives are:




Gather and analyze experimental data using appropriate techniques




Keep record of data using a laboratory notebook and relevant software




Present data using tables and graphs and interpret the findings




Plan research project according to scientific principles and relevant methodologies




Access up to date literature on the scientific question and assess it critically




Perform mammalian tissue culture using appropriate aseptic technique




Perform transfection and gene delivery using viral vectors




Carry out decontamination, disinfection, and biological waste disposal according to laboratory guidelines.




Extraction of RNA, DNA and proteins from cultured cells, tissues and/or patient-derived biological samples




Analyze protein expression using immunofluorescence and/or immunoblotting




Quantify relative gene expression levels using quantitative PCR




Perform molecular cloning to generate DNA vectors for gene expression, gene suppression or gene editing




Prepare samples for DNA sequencing, analyze sequencing results, and use publicly available genomic databases to interpret findings








1 – Students will prepare a presentation focusing upon the work completed. The presentation will be 20 minutes long and will be followed by 5-10 minutes of questions.



2 – Students will keep a laboratory notebook and write a final report describing methodology and results