PBMC culture, cytokine analysis, dendritic cell, T cell, B cell stimulation and interaction, immunoglobulin synthesis and measurement, DNA isolation, PCR, protein electrophoresis and IgE binding to peptides. CD14, TLR4 and TSLP polymorphisms in children with asthma, allergic rhinitis and food allergy. Association of indoleamine 2,3 dioxygenase (IDO) and tryptophan pathway with tolerance development in children with food allergy and Component resolved Diagnosis (CRD) in Allergic diseases. Immunologic mechanisms of IgE-mediated and non-IgE-mediated food allergy with experiments in Caco-2 gut epithelial cell culture. Diagnostic methods and tolerance mechanisms in venom allergy with CRD tests in serum samples, dendritic cell and basophil activation by venom allergens and IDO pathway in venom allergic patients