Duration of the elective: 1 month
Number of students: 1
Location(s): Koç University Rumelifeneri Campus, Koç University Hospital (KUTTAM)
Elective Director: Prof. Dr. Tamer Önder
Elective Faculty: Prof. Dr. Tamer Önder
BRIEF DESCRIPTION OF STUDENT ACTIVITIES
This elective will introduce students to advanced cell and molecular biology techniques used in stem cell biology, tissue culture, cellular reprogramming, and genome editing. Students will formulate a novel hypothesis and test it with appropriate experimentation. Students will gain skills in understanding primary literature, planning experiments with appropriate controls, keeping careful records.
Brief: Students will gain proficiency in generating genome editing tools and stem cell culture.
Specific objectives are:
– Learn the design and cloning of CRISPR-Cas9 based genome editing plasmids
– Create knockout cell lines and verify protein expression via western blot
– Learn derivation and culture of induced pluripotent stem cells
– Reading and critical evaluation of primary research articles
ASSESSMENT OR EVALUATION
1 – Students will prepare a presentation focusing upon the work completed. The presentation will be 20 minutes long and will be followed by 5-10 minutes of questions.
2 – Students will keep a laboratory notebook and write a final report describing methodology and results