BASIC INFORMATION

 

 

Duration of the elective: 1 month

 

 

Number of students: 1

 

 

Prerequisites: None

 

 

Location(s): Koç University Rumelifeneri Campus, Koç University Hospital (KUTTAM)

 

 

 

 

CONTACT INFORMATION

 

 

 

Elective Director: Prof. Dr. Tamer Önder

 

 

Elective Faculty: Prof. Dr. Tamer Önder

 

 

 

 

BRIEF DESCRIPTION OF STUDENT ACTIVITIES

 

 

This elective will introduce students to advanced cell and molecular biology techniques used in stem cell biology, tissue culture, cellular reprogramming, and genome editing. Students will formulate a novel hypothesis and test it with appropriate experimentation. Students will gain skills in understanding primary literature, planning experiments with appropriate controls, keeping careful records.

 

 

 

 

LEARNING OBJECTIVES

 

 

Brief: Students will gain proficiency in generating genome editing tools and stem cell culture.

 

 

Specific objectives are:

 

 

– Learn the design and cloning of CRISPR-Cas9 based genome editing plasmids

 

 

– Create knockout cell lines and verify protein expression via western blot

 

 

– Learn derivation and culture of induced pluripotent stem cells

 

 

– Reading and critical evaluation of primary research articles

 

 

 

ASSESSMENT OR EVALUATION

 

 

 

1 – Students will prepare a presentation focusing upon the work completed. The presentation will be 20 minutes long and will be followed by 5-10 minutes of questions.

 

 

2 – Students will keep a laboratory notebook and write a final report describing methodology and results